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Fig. 4 The TOR pathway is activated in the peri-injury zone during heart regeneration after cryoinjury. (A) Experimental design of the heart regeneration experiment in Tg(cmlc2:dsRed2-nuc) fish with 1 µM rapamycin or 0.1% DMSO treatments. dpci, days post-cryoinjury. (B-D) Immunofluorescence staining of hearts isolated from the experiment shown in A at 7 dpci. The cryoinjured area is outlined with a dashed line. Arrowheads in C indicate PCNA/dsRed2-positive nuclei. n≥5. (E) AFOG histological staining showing the myocardium in orange, fibrin in red and collagen in blue at 30 dpci. n≥5. (F-H) Quantification of data representatively shown in C-E. n≥5. Data are mean±s.e.m.; two-way ANOVA with Šidák multiple comparisons test.