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Fig 2 Microarray data collection, DEG identification and top hits.
(a) Experimental design for tissue collection of whole eyes for microarray analysis. Treatment with Mtz for 24h was induced at 6 dpf following screening for NTR-rod+ or NTR-bipolar+ fish. Eyes were collected in triplicate at the following 12 timepoints including t0, t8, t16, 24, t32, t40, t48, t60, t72, t92, t144 and t240. (b) Venn diagram illustrating the total number of differentially expressed genes (DEGs) unique to either cell type or shared between the two at all timepoints. (c) Chart showing distribution of upregulated and downregulated DEGs in each paradigm. (d) Top 10 up and downregulated genes across the entire data set based on p-value as well as the number of timepoints that gene was identified as differentially expressed out of 12.