Image
|
Figure Caption
Fig. 3 Fiji (ImageJ Ver 2.1.0/1.53c) analysis of the thyroid follicles of a negative control transgenic zebrafish (Danio rerio, tg:mcherry) embryo at ×20 magnification: (A) brightfield (DIA 340 mm) image of the head region; (B) overlay of the DIA and tetramethylrhodamine (TRITC) channel showing the location of thyroid follicles in red; (C) TRITC channel as gray scale used for the image analysis; and (D) thyroid follicle micrograph after image analysis. Three pictures with average intensity were overlaid, and the area within the pink circles was analyzed (D).
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Environ. Toxicol. Chem.