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Fig. 1 Experimental design of exposure experiments with zebrafish. For each control and exposure concentration, three conditions were run: exposure from 0 to 5 days postfertilization (dpf), exposure from 0 to 8 dpf, and exposure from 0 to 5 dpf with subsequent recovery in water until 8 dpf. Three independent replicate runs with three test concentrations of propylthiouracil or tetrabromobisphenol A plus water and solvent control were performed separately for each endpoint. For histopathology, 24 embryos per replicate run and concentration were used. For thyroid follicle analyses, 20 embryos per replicate run and concentration were used. For thyroid hormone measurements, 40 embryos per replicate run and concentration were used. DMSO = dimethyl sulfoxide; PTU = propylthiouracil; TBBPA = tetrabromobisphenol A.