ZFIN Image: Ott et al., 2023, Figure 2

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Figure Caption

Figure 2

Subcellular localization analyses of Clic5 in the pronephric tubule of zebrafish. (A–D) Triple whole-mount immunostaining on 1dpf old Tg(actb2:Mmu.Arl13b-GFP) zebrafish embryos using anti-GFP (labeling Arl13b-GFP, i.e. the ciliary axoneme) (A), anti-γTubulin (labeling the ciliary basal body) (B) and anti-Clic5 (monoclonal antibody produced in mouse) (C) antibodies reveals ciliary localization of Clic5 in the pronephric tubule. (D) Image is (A–C) merged. (E) 3D reconstruction and segmentation of Arl13b-GFP (green), γTubulin (blue) and Clic5 (gold) using Imaris software. Clic5 voxels co-localize with those of ciliary basal body (white arrows) and ciliary axoneme voxels (yellow arrows), respectively. Scale bar, 5 µm.

Acknowledgments

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