Figure 4.

Figure 4. Level of SBDS^R126T protein expression affected embryonic development.

(A) Incross between WT sbds fish with two copies of the transgenic line produced healthy embryos. (B) Incross between sbds KO fish with two copies of the transgenic line produced healthy embryos and deformed embryos. Black star shows healthy embryo, green star shows developmentally delayed, and red star shows deformed embryo. (C) qRT-PCR analysis of critical genes involved in ribosomal, metabolic, and tp53-associated pathways. (D) Western blot showing Eif6 accumulation in zebrafish-deformed embryos. (E) Protein quantification of Western blots as performed with NIH ImageJ. (F) Sudan black staining of 10 dpf larvae to count neutrophils. (G) Standard length and neutrophil number were reduced in sbds KO larvae expressing SBDS^R126T.