Fig 6

Fig 6 Polyploid cells appear transiently in hepatic postembryonic growth in zebrafish.

(A) Whole-mount of a 14-dpf zebrafish liver, displaying sparse multinucleated hepatocytes (N = 1, n = 2 livers; yellow arrowheads indicate binucleated cells). (B) Approximately 5 μm projection of a region of a juvenile liver. Fish SL = 11.16 mm (N = 3, n = 6 livers). (C) Segmentation shows variable nuclear volumes, which correlate with the sum intensity of DAPI, indicating that bigger nuclei have a higher amount of DNA (D). (E) Approximately 5 μm projection of an adult liver region (N = 3, n = 3 livers). (F) Segmented nuclei show only sparse variability in volume, with few bigger nuclei. Nuclear volume correlates with sum intensity of DAPI (G). (H) Schematics representing the transient appearance of polyploid cells over time; blue trajectory is manually approximated based on qualitative analysis. The numerical values that were used to generate the graphs in (D, G, H) can be found in S1 Data. DAPI, 4′,6-diamidino-2-phenylindole; dpf, day postfertilization; SL, standard length.