ZFIN Image: Zhao et al., 2023, FIGURE 6

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Figure Caption

FIGURE 6

Pin1 regulates Nrd stability. Protein stability assay of HA-Nrd in HEK 293T cells stably expressing control siRNA and Pin1 siRNA. (A) HA-Nrd degraded at a higher rate in Pin1 knock-down background in HEK 293T cell line. (B) HA-Nrd5A levels remained stable in Pin1 knock-down background in HEK 293T cells. (C) Overexpression of FLAG-tagged zebrafish Pin1 enhanced Nrd stability. α-tubulin was used as an internal control. (D) Quantification of HA-Nrd levels in (A,C) normalized to α-tubulin levels. (E) Quantification of HA-Nrd levels in (B) normalized to α-tubulin levels. Error bars represent ±SEM.

Acknowledgments

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