Figure 4

Figure 4 Canalicular ultrastructure is disrupted in mtm zebrafish.

Electron microscopy of whole 7 dpf zebrafish was used to define liver ultrastructure. (A) Magnification (original magnification, ×5,000; scale bars: 5 μm) showing multiple hepatocytes, with bile canaliculi outlined by red dashed lines. Fragmentation of mtm canaliculi was already visible at this magnification. (B) Higher-magnification visualization of bile canaliculi (original magnification, ×14,000; scale bars: 2 μm). In the WT panel, canalicular microvilli are apparent (black dashed circle). In the mtm sample, the canaliculus is devoid of microvilli. Green arrows point to fragmented parts of the canaliculus. n = 3 zebrafish per genotype.