FIGURE 4

FIGURE 4

Product-over-time progression curves for α-casein phosphorylation mediated by the zebrafish CK1 isoforms and GST-CK1ε. The time-dependent phosphorylation of α-casein, catalyzed by (A) 70 nM His-DrCK1δA, (B) 33 nM His-DrCK1δB, (C) 7 nM His-DrCK1ε (D) and 70 nM GST-CK1ε was determined in in vitro kinase assays. The ATP-concentration of the reaction mix was 10 μM and 2 g/L substrate were utilized. The assays were performed in presence of the inhibitor solvent DMSO. Linear regression was performed to determine the maximum coefficient of determination (R²) and to identify the initial velocity region (Roth et al., 2021). Product-over-time progression curve for GST-CK1δ was established by Roth et al. (Figures 3, 4) and the data is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0) (Roth et al., 2021).