ZFIN Image: Uttley et al., 2023, Figure 2.

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Figure Caption

Figure 2. HS5 and NRE are active in different zones of the developing retina.

(A) Live imaging at 24, 48, and 72 hpf in the developing retina of NRE-eGFP/HS5-mCherry F1 embryos. D, dorsal; V, ventral; T, temporal; N, nasal. Scale bars 50 μm. (B) Quantification of mean fluorescence intensity for mCherry (HS5) and eGFP (NRE) in the nasal versus temporal retina at 24, 48, and 72 hpf. Each measurement represents one embryo. The activity of HS5 (mCherry signal) is significantly higher in the temporal retina at all time points. n F1 embryos imaged ≥6 for all time points. Wilcoxon test results: ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Acknowledgments

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