ZFIN Image: Tromp et al., 2023, FIGURE 5

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Figure Caption

FIGURE 5

Cre protein injections successfully trigger Lox-recombination. (A–C), Control injections demonstrating no spontaneous Lox-recombination in the absence of Cre protein. (D–R), Injection of 10 pg to 10 ng of cell-permeant Cre protein at one-cell stage. No significant Lox-recombination is observed from 10pg to 100 pg. However, robust conversions are observed from 0.5 ng to 10ng, with 100% of the animal injected with 10 ng presenting total conversion of the Lox-transgenes (i.e., only Red/mKate fluorescence was detected). Note that protein injections may also be an attractive approach for local Lox-recombination at a later stage of zebrafish development. Scale bar 100 µm.

Acknowledgments

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