ZFIN Image: Hagio et al., 2023, Figure 3—figure supplement 2

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Figure 3—figure supplement 2—source data 1. Latency and duration of locomotion induced with channelrhodopsins (ChRs) by light of various light intensities.

Latency (A) and duration (B) of evoked tail movements. The hindbrain area of 3-dpf Tg larvae expressing GtCCR4-3.0, KnChR-3.0, and CrChR2[T159C] and control larvae (non-expressing sibling larvae) were irradiated by light of different light intensities. Six consecutive stimulation trials were analyzed for eight rhodopsin-expressing and non-expressing (control) larvae of each Tg line. One-way ANOVA with Tukey’s post hoc test (latency GtCCR4-3.0-EYFP 0.4 mW/mm² vs. 0.2 mW/mm², p=0.00571; KnChR-3.0-EYFP 0.4 mW/mm² vs. 0.2 mW/mm², p=0.00553; 0.4 mW/mm² vs. 0.1 mW/mm², p=5.26e-06; 0.2 mW/mm² vs. 0.1 mW/mm², p=0.00469). **p<0.01, ***p<0.001, ns: not significant. Means and SEMs are indicated.

Figure 3—figure supplement 2—source data 1.Data for <xref rid='fig3s2' ref-type='fig'>Figure 3—figure supplement 2</xref>, latency and duration of locomotion induced with ChRs by light of various intensities.

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