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Figure 3—figure supplement 2 Latency and duration of locomotion induced with light of various intensities.

Latency (A) and duration (B) of locomotion evoked with light of various intensities. The hindbrain region of 3-dpf larvae expressing SpiRh1, SpiRh1[S186F], and ChRWR in hindbrain reticulospinal V2a neurons was stimulated with light of various intensities (0.4, 0.2, or 0.1 mW/mm2). Six consecutive stimulation trials were analyzed for 4 or 12 larvae of each Tg line (n=12 for SpiRh1 and SpiRh1[S186F], n=4 for ChRWR). One-way ANOVA with Tukey’s post hoc test was used to statistically analyze data. ns: not significant. Means and SEMs are indicated.

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