Fig. 3

Fig. 3 l-CaD expression is upregulated by GR activation promoting growth in organotypic cell culture.

A Correlation between GR and CALD1 mRNA in patient-derived organoids (cBioPortal) [79]. B Western blot depicting GR expression in commercial PCa cell lines. C UCSC genome browser visualization of ReMap ChIP-seq tracks for AR and GR in CALD1 with ENCODE candidate promoter-like signatures highlighted in red and enhancer-like signatures highlighted in yellow and orange. D Barplots depicting l-CaD protein expression in PC3 cells treated with Dex (0.1 µM, 1 µM, or 10 µM) after 24 or 48 hours. The mean and SD of three experiments are shown (ns = not significant, *p < 0.05, and ****p < 0.0001 as determined by t-test). E Barplots depicting l-CaD protein expression in DU145 cells treated with Dex (0.1 µM, 1 µM, or 10 µM) after 24 or 48 hours. The mean and SD of three experiments are shown (ns = not significant, *p < 0.05, and ****p < 0.0001 as determined by t-test). F Barplots depicting l-CaD protein expression in DU145 cells treated with prednisolone (0.1 µM, 1 µM, or 10 µM) after 48 hours. The mean and SD of three experiments are shown (ns = not significant and *p < 0.05 as determined by t-test). G Violin plots of relative spheroid size in PC3 cells transfected with siNeg1 or siCaD-1 and grown in the presence or absence of 1 µM Dex in basement membrane matrix. Boxplot indicates median and whiskers indicate 1.5 times interquartile range pooled form three experiments (ns = not significant, *p < 0.05, and **p < 0.01 as determined by t-test). H Representative brightfield image of day 5 organotypic culture of PC3 cells treated with siNeg-1 or siCaD-1 and exposed to 1 µM Dex or vehicle (DMSO) on day 3 of culture in basement membrane matrix.