Figure 6

Figure 6

(A) FPALM image showing Dystroglycan-Dendra2 localizations in whole zebrafish muscle fiber and (B) its corresponding brightfield image. (C) Dystroglycan–Dendra2 localizations are seen in the MTJ, sarcolemma, and t-tubule regions without density thresholding. (D) Localizations thresholded to those areas above the average localization density (localizations per μm²). (E) Localizations after thresholding at or above three times the average density show few remaining at the t-tubule, indicating lower density in the t-tubule region. (F) The remaining localizations after thresholding at or above five times the average density indicate that the MTJ is the most dense region for localizations, followed by the sarcolemma.