Figure 3

Figure 3

(A) Confocal image of a 72 hpf zebrafish antibody-stained for Dystroglycan, with Dystroglycan seen mainly at the myotendinous junction (MTJ), indicated by arrows. (A1) Confocal image shows Dystroglycan at both the MTJ and sarcolemma surrounding individual muscle fibers. (B) Confocal image shows transgenically expressed Dystroglycan–GFP is present at the (B3) MTJ, (B2) sarcolemma membranes, and (B1) t-tubules. (C) Confocal image of Dystroglycan-Dendra2 muscle fiber in green, (C1) merged Dystroglycan-Dendra2 muscle fiber in green with ryanodine receptor on t-tubules in magenta, and (C2) merged Dystroglycan-Dendra2 in green with phalloidin staining of actin in cyan (D) Confocal image of 48 hpf muscle fiber expressing Dystroglycan-Dendra2 fairly continuously at MTJ and t-tubule. (E) Super-resolution, FPALM image render, of 48 hpf muscle fiber expressing Dystroglycan-Dendra2, demonstrates that individual molecules can be localized and Dystroglycan can now be seen as clusters at the MTJ and t-tubule. Magnifications in the green boxed regions highlight the differing levels of information about the distribution of Dystroglycan at the MTJ that can be obtained from confocal and super-resolution images.