Figure 3

Figure 3

Loss of Klf9 function affects expression of per1a and crystallin genes only at a specific time of day. (a) Correlation of per1a expression variance with PCs 2–4. (b) Mean normalized expression of per1a at each time point in each genotype; error bars indicate the SEM. (c) klf9^−/− induced differential expression of per1a with respect to the DESeq2 distribution at each time point; *Adjusted p-value = 0.0013. (d) Nanostring analysis of per1a expression with respect to time over an 8-h time course. Each sample consisted of total RNA pooled from 4–5 larvae collected at the indicated times on 5 dpf (see Gans et al., 2021, ref.¹⁷). (e) klf9^−/−-induced differential expression of 25 cry genes with respect to the distribution of all genes that are differentially expressed between klf9^−/− and WT at each time point. (f) Temporal profiles of cry gene expression. Error bars are the SEM of the four biological replicates.