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Fig. 2

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ZDB-IMAGE-230729-3
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Figures for Lei et al., 2023
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Fig. 2

Hcy promotes MKs differentiation via GH-PI3K-Akt axis. A Venn diagram visualizing the elevated Pathway Interaction Database pathways of MKs subpopulations in Hcy group. B Heatmap showing the relative GSVA scores for each gene set based on bulk RNA-seq of BM MKs (n = 4). C and D Western blot analysis of p-PI3K and p-AKT (Ser473) in Meg-01 cells after exposure to Hcy (100 μM) for indicated time period. Total PI3K, AKT and GAPDH were used as loading control. Significance according to one-way ANOVA with LSD multiple comparisons test (n = 3). E Culture-derived MKs and F platelets were analyzed by flow cytometry. Significance according to one-way ANOVA with Tukey multiple comparisons test (n = 3). G PLT in Ghr−/− mice. Significance according to two-tailed unpaired t test (n = 6). H Quantification of CD41+ MKs in femurs bone marrow of Ghr−/− mice. Significance according to Mann–Whitney test (n = 6). I and J Western blot analysis of p-PI3K and p-AKT (Ser473) in the bone marrow MKs after exposure to Hcy (100 μM) for 30 min. Significance according to one-way ANOVA with Tukey multiple comparisons test (n = 6). K Bar diagram showing the percentage of MK2 to all cells. L Peripheral PLT. Significance according to one-way ANOVA with Tukey multiple comparisons test (n = 8). M Representative images and N quantification of CD41+ MKs (green) in femurs bone marrow of mice. Scale bars, 50 μm. Significance according to one-way ANOVA with Tukey multiple comparisons test (n = 8). O Bar chart showing the significantly down-regulated GO-BP terms and P PID pathways in MKs. T values are from the linear model in the limma package. QR Western blot analysis the level of p-PI3K and p-AKT (Ser473) in Meg-01 cells after exposure to Hcy (100 μM) with or without MT (1 μM) for 30 min. Significance according to one-way ANOVA with Tukey multiple comparisons test (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Hematol. Oncol.