Fig. 2

Fig. 2

Figure 2. In vivo and in vitro screens. (A) Zebrafish embryos were exposed to several concentrations of different PFASs from ∼6 hpf to 96 hpf. At 96 hpf, larvae were assessed for developmental toxicity or respiratory burst. (B) HL-60 cells were differentiated to a neutrophil-like phenotype (nHL-60) via DMSO treatment for 5 days. At the end of this period, cells were dosed with PFASs and plated into a 96-well plate. At 96 hr post plating, cells were assessed for cytotoxicity or respiratory burst. This strategy was also employed for a 24-hr PFAS exposure. Panel A is adapted from Phelps et al. (2020 Phelps D, Fletcher A, Rodriguez-Nunez I, Balik-Meisner M, Tokarz D, Reif D, Germolec D, Yoder J. 2020. In vivo assessment of respiratory burst inhibition by xenobiotic exposure using larval zebrafish. J Immunotoxicol. 17(1):94–104.[Taylor & Francis Online], [Web of Science ®] , [Google Scholar]). Neutrophil images in Panel B were acquired from BioRender.