Fig. 4

Fig. 4 Fig. 4. Effects of prolonged exposure of larvae-juvenile AB zebrafish from 15 dpf – 7 mm to 10 ng/L EE2. Representative lateral and ventral images of Alcian blue and Alizarin red stained juvenile zebrafish in control and EE2 treated groups (A). Plot showing weight (mg) of individual juvenile zebrafish in control and EE2 treated groups (B). Middle black line indicates the mean, and the upper and lower lines indicate the 95 % confidence interval of the mean, * ** p ≤ 0.001. Principal component analysis of juvenile zebrafish craniofacial structures in each treatment group (C). Wireframe graphs are shown on each axis. Orange indicates the mean shape and blue indicates the shape change in a positive direction of the relevant PC. Plot of PC1 and weight (mg) in juvenile zebrafish to assess correlation between jaw phenotype and weight (D).