Fig. 2

Fig. 2 Fig. 2. Effects of exposure of AB zebrafish embryo-larvae to either 10 ng/L or 1 µM EE2 from 15 hpf–5 dpf on weight and craniofacial structure. Weight (mg) of all larvae in control and EE2 treatment groups (50 per group, n = 4) (A). Representative lateral and ventral images of 5 dpf Alcian blue stained zebrafish in control, 10 ng/L EE2, and 1 µM EE2 groups (B). Principal component analysis of zebrafish larvae craniofacial structures in each treatment group (C). Wireframe graphs are shown on each axis. Light grey indicates the mean shape and dark grey indicates the shape change in a positive direction of the relevant PC.