Fig. 1.

Fig. 1.

Evaluation of Ac/Ds and Tol2 transposition in F0 embryos. (A) Schematic of enhancer construct containing enhancers (‘enhancer’) upstream of E1b minimal promoter (‘E1b-mp’) driving eGFP expression. Two versions of each enhancer construct (with Ds- or Tol2-integration arms) were tested by microinjection into one-cell-stage embryos. Ac or Tol2 clutches of the same enhancer were imaged using the same settings on a fluorescent stereomicroscope. Embryos with the same expression pattern (Table S1) were counted. Scale bars: 500 µm. (B) Ds- or Tol2-armed pax3a enh5 was microinjected with Ac or Tol2 mRNA, respectively, into Gt(FoxD3:mCherry)^ct110aR embryos to visualise the neural tube (mCherry, cyan). Live confocal imaging highlighted similar levels of neural crest cell labelling (eGFP, yellow) between 30 pg Ds and 150 pg Tol2 vector DNA, but 30 pg Tol2 vector DNA yielded a visually weaker signal. Scale bars: 50 µm.