Fig. 3

Fig. 3 Network analysis of candidate suppressors of mHTT toxicity.

a Bubble plot representing the number of independent integration events for each NGS hit (1356 hits, represented by bubbles and ordered along the x axis in alphabetical order). Size and colours of the bubbles are related to the number of independent screening experiments in which they were found. Labels indicate relevant candidate genes and additional genes belonging to the same families. b The interactome, realised with Cytoscape¹⁴¹, shows physical and regulatory interactions of over-activated genes in mHTT-resistant clones with HD related genes, retrieved through HDNetDB⁵⁶, and genes identified from NGS screening. Main nodes correspond to the mutated genes of HTT-resistant clones (violet label), HD related genes are marked with a blue label and hits from the NGS screening are represented with green labels. c Enrichment analysis of the genes represented in the interactome (Fig. 3b) based on HD gene sets using HDNetDB. Statistical enrichment and p-values were calculated using the hypergeometric distribution.