IMAGE

FIGURE 1

ID

ZDB-IMAGE-230630-4

Genes

EGFP
mCherry

Antibodies

Ab3-dag1

Publication

Hipke et al., 2023 - Loss of TDP-43 causes ectopic endothelial sprouting and migration defects through increased fibronectin, vcam 1 and integrin α4/β1

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Figures for Hipke et al., 2023

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FIGURE 1

Loss of TDP-43 leads to increased vascular sprouting and defective migration. (A) More and ectopic SA sprouts in zebrafish tardbp−/−; tardbpl−/− mutants. Immunofluorescence of a sibling and a tardbp−/−; tardbpl−/− mutant embryo at 24 hpf. The Tg (fli1a:EGFP)^y1 highlights the vasculature in green, the somite boundaries are stained in red. Scale bar 50 μm, anterior to the left. The insets a and b highlight the vasculature only. DA = dorsal aorta (B) Impaired directed migration in tardbp−/−; tardbpl−/− mutants. Less mutant EC nuclei have reached the DLAV at 32 hpf. Embryos are transgenic for Tg (fli1:nlsEGFP)^y7 with nuclear EGFP expression (green) and Tg (kdrl:HsHRAS-mCherry)^s896, highlighting the vasculature (red). Scale bar 50 μm, anterior to the left. The insets a and b highlight the EC nuclei (left) and the vasculature (right). (C) Quantifications of the vascular defects in the tardbp−/−; tardbpl−/− mutants as indicated. Kruskal–Wallis test and Dunn’s multiple comparison post test, n ≥ 9, sprouts between boundaries of six somites dorsal to the end of the yolk sack extension were quantified. D’Agostino-Pearson omnibus normality test and unpaired t-test, n = 10 for "% EC contributing to DLAV” and “number of EC in DLAV of 4 segments at 2.5 dpf”.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol