Figure 4

Figure 4

Heterogenous dynamic morphologies of LC3-positive Mm-containing vesicles. Transgenic (CMV:GFP-LC3) zebrafish larvae, labeling LC3 autophagy-related protein (green), were infected with 100 CFU of Mm-mCherry (pseudo color magenta) following the TFI protocol. (A) Maximum projection stills of an infected macrophage that reshapes an LC3-positive Mm-containing vesicle (arrowhead) during migration (60–65 mpi) (Supplementary Video S5). Note how the Mm-containing vesicle (arrowhead) is surrounded by puncta (yellow arrow) and tubular (white arrow) LC3-positive structures and subsequently changes into an elongated form (arrowhead, 62–64 mpi), then reshapes (65 mpi). A zoomed-in Z stack selection of the LC-positive structures at 66 min (outlined yellow) is shown to reveal the presence of Mm. (B) Orthogonal view of the complete Z-stacks, stills capturing the moment when an Mm cluster (white arrowhead) is incorporated into an LC3-positive spacious vesicle (88–93 mpi), after which the vesicle reshapes to a compact form (102–159 mpi) (Supplementary Video S6). Scale bars: 10 μm for all maximum projection stills in A and B, and 5 μm for the zoomed-in area in A and the orthogonal views in B.