FIGURE 2

FIGURE 2

Visual impairment in the rpgra^−/− larval zebrafish. (A) Detection of visual function by ERG analysis of WT zebrafish at 5 dpf. (B) Detection of visual function by ERG analysis of rpgra^−/− zebrafish at 5 dpf. (C) Comparison of b-wave amplitudes between WT (n = 5) and rpgra^−/− (n = 6) zebrafish using two-tailed Student’s t-test. The result is shown as mean ± SD. **, p < 0.01. (D–F) Histological analyses of WT, rpgra^+/−, and rpgra^−/− zebrafish at 5dpf. Cryosections were stained with hematoxylin and eosin. (D′–F′) Enlarged images of the area inside the yellow box in the (D–F). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; L, Lens. Scale bars, 50 µm.