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Fig. 2 aPKCλ and Pals1a are not required for Schwann cell proliferation during radial sorting. PH3 immuno-labeling in Tg(foxd3:gfp) (A-A″), Tg(foxd3:gfp)/has−/− (B-B″), and Tg(foxd3:gfp)/nok−/− (C-C″) embryos at 48 hpf. Arrows indicate SC that are GFP and PH3 positives. Scale bar = 25 μm. (D) Quantification of the total number of SC within a defined region of the PLLn at 48 hpf in control Tg(foxd3:gfp) (44.91 ± 1.46 cells, n = 11 embryos), has−/−/Tg(foxd3:gfp) (46.91 ± 2.70 cells, n = 11 embryos), and nok−/−/Tg(foxd3:gfp) (48.20 ± 2.21 cells, n = 10 embryos) embryos (P > .79 in all cases). (E) Quantification of the number of PH3+/SC within a defined region of the PLLn at 48 hpf in control Tg(foxd3:gfp) (1 ± 0.40 cells, n = 11 embryos), has−/−/Tg(foxd3:gfp) (1.36 ± 0.47 cells, n = 11 embryos) and nok−/−/Tg(foxd3:gfp (1 ± 0.29 cells, n = 10 embryos) embryos (P > .79 in all cases). (F) Quantification of the ratio of PH3+ SC relative to the total number of SC within a defined region of the PLLn at 48 hpf in control Tg(foxd3:gfp) (0.02 ± 0.008, n = 11 embryos), has−/−/Tg(foxd3:gfp) (0.028 ± 0.009, n = 11 embryos), and nok−/−/Tg(foxd3:gfp (0.022 ± 0.006 cells, n = 10 embryos) embryos (P > .75 in all cases). (G) Still images from time-lapse imaging in Tg(foxd3:gfp), Tg(foxd3:gfp)/has−/−, and Tg(foxd3:gfp)/nok−/− embryos at around 50 hpf. Arrows indicate Schwann cells prior to and after cytokinesis. (H) Quantification of the time required for control (average of 9.30 ± 0.61 min, 20 cells, n = 4 embryos), has−/− (average of 11.15 ± 0.57 min, 34 cells, n = 7 embryos) and nok−/− (average of 9.79 ± 0.50 min, 24 cells, n = 6 embryos) Schwann cells to successfully complete cytokinesis. (ns, P > .05 in all cases). min, minutes. (I) Quantification of the angle measured along daughter Schwann cells during cytokinesis relative to axon position in control (average of 176.8 ± 0.58, 24 cells, n = 4 embryos), has−/− (average of 174.60 ± 0.92, 22 cells, n = 5 embryos), and nok−/− (average of 176.10 ± 0.79, 19 cells, n = 6 embryos) embryos (ns, P > .85 in all cases)