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Fig. 1 Pals1a and aPKCλ are not required for axonal growth or Schwann cell migration. (A) Acetylated tubulin expression in control (n = 12 embryos), has−/− (n = 12 embryos), and nok−/− (n = 11 embryos) embryos at 48 hpf showing the PLLn nerve (arrows). Scale bar = 100 μm. Still images from time-lapse imaging in Tg(foxd3:gfp) (B-B″') (5 cells, n = 3 embryos), Tg(foxd3:gfp)/has−/− (C-C″') (5 cells, n = 4 embryos), and Tg(foxd3:gfp)/nok−/− (D-D″') (6 cells, n = 3 embryos) embryos at around 32 hpf. Arrows indicate Schwann cells prior to and after cytokinesis. Scale bar = 25 μm. min, minutes. (E) Quantification of the velocity of Schwann cells in controls Tg(foxd3:gfp) (1.77 ± 0.05 μm/min, 12 cells, n = 4 embryos), Tg(foxd3:gfp)/has−/− (1.59 ± 0.04 μm/min, 15 cells, n = 4 embryos), and Tg(foxd3:gfp)/nok−/− (1.73 ± 0.07 μm/min, 11 cells, n = 4 embryos) during their migration along the PLLn between 28 and 34 hpf (P > .062 in all cases). ns, non-significant