ZFIN Image: Zeng et al., 2023, Figure 4

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Figure 4

Deletion of Tarsl2 exerts no effect on MSC integrity or tRNA aminoacylation in vivo.A, protein levels of various MSC components in the 6-week-old Tarsl2^+/+ and Tarsl2^−/− mouse muscle. B, incorporation of several MSC components in the 6-week-old Tarsl2^+/+ and Tarsl2^−/− mouse muscle lysates. C and D, levels of tRNAs [tRNA^Lys(CUU), tRNA^Ile(AAU), tRNA^Gln(CUG), tRNA^Arg(ACG), and tRNA^Leu(AAG)] in the 6-week-old Tarsl2^+/+ and Tarsl2^−/− mouse muscle. E and F, aminoacylation levels of tRNA^Lys(CUU) and tRNA^Ile(UAU) in the 6-week-old Tarsl2^+/+ and Tarsl2^−/− mouse muscle. De: tRNA deaminoacylation control. G and H, global protein synthesis rate of the 6-week-old Tarsl2^+/+ and Tarsl2^−/− mouse MEFs, as determined by puromycin incorporation assay. Gapdh was included as the loading control. Error bars in (D, F, and H) represent the mean ± SD with the p values indicated (two-tailed Student’s t test). MEF, mouse embryonic fibroblast; MSC, multiple tRNA synthetase complex.

Acknowledgments

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