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Figure 5
Stability assay shows more perdurance of O.alcalica MyoD1 compared to D. rerio MyoD1. (A) Western blot analysis of extracts from Xenopus laevis explants overexpressing HA-tagged MyoD1 proteins. Explants were incubated for either 0 (T0), 1 (T1) or 3 h (T2) in the translational inhibitor, cycloheximide. Anti- beta tubulin is a loading control. (B) Quantification of the loss of protein relative to the amount of protein detected at T0 is shown in a graph for O.alcalica MyoD1 and for D. rerio MyoD1.
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