Figure 5—figure supplement 3.

Figure 5—figure supplement 3. Mutation of vsx genes in medaka impairs INL differentiation and eye growth.

(a) CRISPR/Cas9 was used to eliminate (green box) the DBD from vsx1 (top) and vsx2.1 (bottom) TFs in medaka. Blue boxes represent exons, black boxes the location of sgRNAs used and primers for screening are depicted as opposing arrowheads. (b-e). Histological sections from WT (b, d, n=4) and vsxKO central retinas (c, e, n=5) at 12dpf. ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer, hpf: hours post-fertilization, dpf: days post-fertilization. Scale bar (b-c): 50 µm, (d-e): 20 µm.