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Figure 1.
(A,B) Shown are the representative drawing of the PHA/PHB sensory neuron (phasmid neurons located in the tail). Cilia, dendrites, axons, and cell soma (cell body) are depicted in the drawing. Fluorescence images from the transgenic strain carrying WDR-31::GFP or GFP::ELMD-1 were displayed in the PHA/PHB sensory neurons. Scale bars: 3 μm. (C, D) Co-localization of WDR-31::GFP (Green) with the IFT-140::mCherry (Red, an IFT-A component, a ciliary marker) or TBG-1:mKate (Red, γ-Tubulin, the basal body) in the tail (phasmids) and head (amphid) sensory neurons. (E, F) TZ and BB denote the transition zone and the basal body, respectively (E, F) GFP::ELMD-1 (Green) localizes to the BB and PCMC (the periciliary membrane compartment) proximal to the transition zone. Co-labelling of GFP::ELMD-1 with MKS-6:mCherry marker (transition zone) or IFT-140::mCherry in the tail (phasmids) and head (amphid) neurons. Scale bars: 2 μm. (G) Shown are representative localizations of WDR-31 and ELMD-1.