ZFIN Image: Lv et al., 2023, Fig. 4

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Figure Caption

Fig. 4

Depletion of mecom impairs zebrafish blood vessel formation.

a CRISPR-cas9 target site on mecom gene. The target sequence is based on the anti-sense strand. b DNA sequencing of mecom mutant animals. The PCR product covering the designed mutation site was cloned into T-vector and the individual colony harbored the insert was sent for DNA sequencing. The TC → CCACCA indel causing frameshift in mecom gene is highlighted. The vertical bars demarcate the mutation site. c mecom gene disruption produces a truncated protein. d Relative gene expression levels of mecom. e Western blot to show Mecom protein levels. f Confocal imaging of intersegmental vessel (ISV) at 30 hpf (top) and subintestinal vein (SIV) at 3 dpf (middle) as well as alkaline phosphatase staining of SIV at 3 dpf (bottom) of mecom mutant and wild type control. g, h Quantification of ISV length (g) and SIV length (h). i Percentage of larvae showing normal or inhibited SIV sprouting. Scale bars, 100 μm. Data are presented as mean values ± SD; n = 6 (d) and n = 4 (e) biologically independent samples, numbers of biologically independent animals analyzed are indicated in the bars of (f–i). P values are determined by two-tailed Student’s t test. Source data are provided as a ^{Source Data file}.

Acknowledgments

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