ZFIN Image: Shoenhard et al., 2022, Fig. 5

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Figure Caption

Fig. 5

Multivariate analysis of variegated expression in neurons (MAVEN) identifies a hindbrain region where CaSR overexpression correlates with decision-making phenotype

(A) Experimental workflow for MAVEN experiment.

(B) Location of the dorsal cluster rhombomere 6 (cyan), relative to the Mauthner soma (purple), in rhombomere 4. Image generated using ZBrain 2.0’s 3D Viewer tool (https://zebrafishatlas.zib.de/).

(C) Normalized fluorescence intensity signal in the dorsal cluster rhombomere 6 in brightly expressing αtubulin:Gal4; UAS:CaSR-EGFP; CaSR^p190/+ larvae of various CaSR genotypes that were SLC-shifted in response to a weak, primarily LLC-evoking stimulus. Two-way ANOVA column factor (phenotype) p < 0.0001. Non-shifted vs. escape-shifted wild-type larvae p = 0.0462. Non-shifted vs. escape-shifted mutant larvae p = 0.0027. n.s. indicates p > 0.05. Multiple comparisons were controlled using Sidak’s multiple comparison’s test. Lines represent mean +/− SEM.

Acknowledgments

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