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Fig. 2 Visual motor response behavior assay in zebrafish larvae at 120 hpf following an embryonic exposure (1–72 hpf) to PFOA (A, D, G), PFHxA (B, E, H), or PFBA (C, F, I). N = 8 biological replicates with 13–16 subsamples per treatment per replicate to total 122–128 fish per treatment group. Error bars represent standard deviation. *p < 0.05 compared to the control treatment group within each phase based on lsmeans table when a significant ANOVA for treatment or interaction occurred. (D1: first dark phase; L1: first light phase; D2: second dark phase; L2: second light phase; D3: third dark phase).
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