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Figure 2
Muscle birefringence of TSA-treated zebrafish larvae following manual or pronase dechorionation. (A–H) Zebrafish trunk skeletal muscle birefringence at 4 dpf. Lateral views, anterior to the left. Images show (A,E) dmd+/+ wild-type (WT) + DMSO, (B,F) dmd+/+ WT + TSA, (C,G) dmd−/− (dmd) + DMSO, and (D,H) dmd−/− (dmd) + TSA. (I) Graph of the average normalized birefringence pixel intensities (mean grey values) in WT (dmd+/+) and dmd (dmd−/−) zebrafish larvae treated with DMSO (control), and dechorionated with forceps (left) or pronase (right). Horizontal lines represent the mean and standard error of the mean. Each dot represents an individual embryo (n = 18–31 animals per condition). Mean gray values between dmd + DMSO and dmd + TSA animals were significantly different for each dechorionation condition. The mean grey values between dmd + TSA animals were not significantly different when comparing each dechorionation condition. Error bars represent the standard error. Significance was determined using a one-way ANOVA test comparing each treatment group to the dmd DMSO control group with Dunnett’s correction for multiple comparisons. Data in support of (I) is available in Table S1 in Supplementary Materials.