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Fig. 4 585858a Transgenic kdrl:EGFP zebrafish embryos were treated with SGLT2 inhibitors from 23 hpf to 54 hpf and fixed. Fixed embryos were stained for nuclei with methyl green. The samples were imaged with light-sheet fluorescence microscope. The endothelial nuclei were computationally identified through colocalization between general nuclear signal and vascular EGFP signal. Representative images are shown. Scale bar 100 µM.(4b) Quantification of the number of endothelial nuclei in ISVs. DMSO, n = 4 embryos (34 ISVs); EMPA (50 µM empagliflozin), n = 4 embryos (39 ISVs); DAPA (50 µM dapagliflozin), n = 3 embryos (23 ISVs); CANA (50 µM canagliflozin), n = 3 embryos (30 ISVs). Embryos from the same set as in experiments in Fig. 3 were used in the analysis. Statistical analysis was performed with one-way ANOVA and Dunnett´s post hoc test. **P < 0.01, ns=non-significant.