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Fig. 1.
Aging reduces the regeneration of zebrafish caudal fin. Old (4 years) and young (4 months) male zebrafish were subjected to caudal fin regeneration assay. (A) Microscopy images of fins before, right after and 8 days after resection. Scale bar: 1 mm. (B) Quantification of the total regeneration of the caudal fin. Two-way ANOVA with age and time as factors was used for statistical analysis. All time points were used in the statistical analysis. (C) Quantitation of thickness of regenerating area. Two-way ANOVA with age and time as factors was used for statistical analysis. All time points were used in the statistical analysis. (D,E) Comparison of the regeneration in dorsal and ventral part of caudal fin. Data from young fish in panel D and in old fish in E. n=12 in both age groups. Two-way ANOVA, with age and anatomical location as factors, was used for statistical analysis.