ZFIN Image: Wang et al., 2023, Fig. 5

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Figure Caption

Fig. 5

Single-cell RNA sequencing revealed differentiation blockade and enrichment of leukemia-associated gene signatures in FLT3^ITD and IDH2 double mutant zebrafish.

A Lineage differentiation trajectory and pseudotime (B) analysis for the erythroid lineage, with HSPC-MPP as the starting point. C Quantification of the pseudotime values of the erythroid progenitor and erythrocyte (D) in the double mutant and wildtype zebrafish. E Lineage differentiation trajectory and pseudotime (F) analysis for the myeloid lineage, with HSPC-MPP as the starting point. G Quantification of the pseudotime values of the myeloid progenitor and neutrophil (H) in the double mutant and WT zebrafish. I Differentially enriched Hallmark and C2 pathway signatures within the HSC, HSPC-MPP, myeloid, and erythroid progenitor clusters between Tg(Runx1:FLT3^ITDIDH2^R140Q) and WT or Tg(Runx1:FLT3^ITDIDH2^R172K) and WT. Wilcoxon Rank Sum test was performed for C, D, G, and H, ****P < 0.0001.

Acknowledgments

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