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Figure 4
Intracellular localization of 3 (25 µM) using melanoma cells (518A2) after 10 min. The uptake was visualized using a Cu(I)-catalyzed reaction with 3-azido-7-hydroxycoumarin (white), and the nuclei were counterstained (Nuclear Green, blue). The right image shows the magnified section marked with a white box. The experiment was carried out in duplicate. The scale bar corresponds to 100 µm (left) or 25 µm (right), magnification of 630×.
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