ZFIN Image: Eeza et al., 2023, Fig 1

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Fig 1

Magnetic resonance anatomical imaging and selective fat imaging in control (Ctr) and lepb-deficient (lepb^-/-) adult zebrafish.

(A) Representative sagittal MR images of female (♀) and male (♂) Ctr and lepb^-/- zebrafish measured by RARE pulse sequence (TE = 15 ms with effective TE of 33.6 ms; TR = 2000 ms; ns = 8; Scan time 17 min). Scale bar: 1 mm. (B) Representative coronal MR images showing muscle area of female Ctr and lepb^-/- zebrafish measured by RARE pulse sequence. Scale bar: 500 μm. A zoomed view (right column) showing fat infiltration in muscles of lepb^-/- zebrafish. Scale bar: 250 μm; (C) Histological sections from the same muscle region as in (B) stained with HE staining. Scale bar: 250 μm. (D) Images of fat distribution in the sagittal plane, acquired with Chemical Shift Selective (CHESS) pulse sequence in female Ctr and lepb^-/- zebrafish. Scale bar: 1 mm; (E) Quantification of body fat in control and lepb^-/- female and male adult zebrafish measured from CHESS MR images (n = 6 per group) (***p < 0.001); (F) Quantification of fat in muscle region from control and lepb^-/- female and male adult zebrafish measured from CHESS MR images (n = 6 per group) (***p < 0.001).

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