ZFIN Image: Baillie et al., 2023, FIGURE 3

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Figure Caption

FIGURE 3

Micro-cannula placement and measurement of atrial dilation and functional effects. (A) In vivo image of the 48 hpf zebrafish heart expressing eGFP (Tg(myl7:eGFP) and the micro-cannula filled with fluorescein, showing the relative position of the cannula to various cardiac regions. (B) Videos of heart during atrial dilation were analysed in Matlab to calculate function parameters at baseline immediately before (B1-B3) and at the end of load application (S1-S3). Atrial end-diastolic (EDA) and end-systolic (ESA) area were measured by tracing the area of the atria at the end of filling and the end of ejection, respectively, from which stroke area (SA) was calculated as EDA - ESA. (C) HR was measured from the time between peaks of filtered image intensity signals, acquired by averaging intensity within a region of interest placed over the atrial wall (red boxes in B).

Acknowledgments

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