|
Fig. 13 Effect of misexpression of sp5a and sp5l on sensory and neural development. (A) Dorsolateral views showing expression of pax5, gsc, and neurog1 at 24 hpf following serial activation of hs:sp5a or hs:sp5l at 12 hpf and 14 hpf. Scale bar (Aa), 50 μm. (B, C) Quantification of the mean number of gsc + cells counted in wholemount embryos (B) and the mean number of neurog1+ cells in the otic vesicle counted from serial sections. Embryos were exposed to a single heat shock at 12 hpf (1HS) or serial heat shocks at 12 hpf and 14 hpf (2HS), as indicated in the key at the top. Error bars (standard deviations) and sample sizes are shown for each group. Asterisks indicate a significant difference from control embryos. (D) Summary of regulation of sensory-neural patterning by Fgf and Wnt. Gray arrows show regulation by Fgf and/or Wnt of medial factors pax2a, sp5a and sp5l, which help upregulate and maintain their own expression and promote aspects of sensory development, and are required to repress neurogenesis otherwise activated by Fgf.
Reprinted from Developmental Biology, 492, Tan, A.L., Mohanty, S., Guo, J., Lekven, A.C., Riley, B.B., Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear, 139-153, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.