Image
|
Figure Caption
Fig. 4
Genotyping wild-type, heterozygous and homozygous zebrafish with 7 bp-insertion in vwf.
(a) The expected DNA sequences of the wild-type (wt) and mutant (mu) (i.e., 7 bp-insertion) in zebrafish von Willebrand factor (vwf). (b–c) are the T7E1 digestion patterns of the PCR products in the absence (−) and the presence (+) of a wt PCR product, respectively. Here, * and # indicate the presence of the cleavage bands (arrowheads) in the absence and presence of a wt PCR sequence, respectively. (d–e) Sanger sequencing results confirmed the genotyping results (+7 bp-insertion) revealed by the agarose gel electrophoresis following the T7E1 digestion.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ J Clin Transl Pathol