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Fig. 3
Genotyping wild-type, heterozygous, and homozygous zebrafish with a 2 bp-deletion in ankrd26.
(a) The expected DNA sequences of the wild-type (wt) and mutant (mu) (i.e., 2 bp-deletion) ankrd26. (b–c) The T7E1 digestion patterns of the PCR products in the absence and presence of a wt PCR product, respectively. Here, * and # indicate the presence of the cleavage bands (arrowheads) in the absence (−) and the presence (+) of a wt PCR sequence, respectively. (d–e) Sanger sequencing results confirmed the genotyping results (−2 bp) revealed by the agarose gel electrophoresis following T7E1 digestion.
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