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Fig. 2
Genotyping wild-type, heterozygous, and homozygous 8 bp-deletion mutation in zebrafish adamts13.
(a) The expected wild type (wt) and mutant (mu) (8-bp deletion) sequences of zebrafish adamts13. (b–c) are the T7E1 digestion patterns of the PCR products in the absence (−wt) and presence (+wt) of the wt PCR product, respectively. Here, * and # indicate the presence of the cleavage bands (arrowheads) in the absence and presence of a wt sequence, respectively. (d–e) show the Sanger sequence tracings of wt and mu (−8 bp), respectively.
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