ZFIN Image: Zhang et al., 2022, Fig. 1

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Figure Caption

Fig. 1

nde1 Expression analysis and generation of nde1 deficiency zebrafish

(A) Relative expression of nde1 in zebrafish embryos during early development. Data are represented as mean ± SEM.

(B) Relative expression of nde1 in zebrafish brain during five dpf to six mpf.

(C–G) Whole mount in situ hybridization of nde1 during early development. Scale bar: 0.2 mm.

(D′) Amplified images of boxed area in (D).

(H) Diagram of target site on second exon of zebrafish nde1 genomic DNA, representative sequencing results and predicted ORF sequence of nde1^+/+ and nde1^−/−; red letter indicates N20 sequence; blue letter indicates NGG.

(I) Reduced expression of nde1 mRNA in the brain of 6 mpf nde1^−/− zebrafish. ∗∗∗p < 0.001. Data are represented as mean ± SEM

Acknowledgments

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