Fig. 6

miR-223-3p association with abnormalities in the zebrafish eye. Histological examination was performed on coronal sections of whole zebrafish larvae that were stained with hematoxylin and eosin. Both miR-223-3p mimic and hyperglycemia conditions displayed abnormal eye morphology and eye cellular components, including the ganglion cell layer (GGL), inner nuclear layer (INL), and outer nuclear layer (ONL). The MiR-223-3p overexpression resulted in sparse cellular arrangements within the eye compared to matched glucose condition. All other groups demonstrated severe abnormalities in the cellular components of the eye. Images were obtained using a Philips slide scanner at ×40 magnification