Figure 4

Metabolic profiles of tlr2 mutant zebrafish larvae measured by NMR and analysis by PLS-DA and heatmap clustering. (A) The representative one-dimensional ¹H NMR spectra of tlr2 wild type and mutant zebrafish larvae measured by NMR spectrometry. Spectra from chemical shift 0.8 to 4.3 were assigned to specific metabolites. Thr threonine, Lac lactate, Asp aspartate, Ser Serine, tCr total creatine (creatine + phosphocreatine), Glc Glucose, 2-Pho 2-Phosphoglycerate, Ala alanine, Gln glutamine, Arg arginine, Leu leucine, Val valine, Gly glycine, Tau taurine, His Histidine, Tyr tyrosine, Mal Malate, Met Methionine, Suc Succinate, Pyr Pyruvate, Glu glutamate, Acet Acetate, Lys lysine, Mel Melatonin, Eth Ethanol, Isol Isoleucine, 2h3m 2-Hydroxy-3-methylvalerate. (B) PLS-DA analysis of tlr2 wild type and mutant zebrafish larvae groups. N = 3, each replicate represents 120 pooled larvae. (C) Heatmap analysis of 57 metabolites detected in tlr2 wild type and mutant groups. N = 3, each replicate represents 120 pooled larvae. FC represents fold change in tlr2^−/− versus tlr2^+/+ zebrafish larvae. For more detailed information on significantly differentially expressed metabolites see Table S5.